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Record: 73

Category: Column Selection
Product:
Sample: partially-hydrolyzed polyacrylamide(HPAM) and some hydrophobically-modified HPAMs

Question
Our group would like to evaluate the MW and MWD of partially-hydrolyzed polyacrylamide(HPAM) and some hydrophobically-modified HPAMs. The HPAM of interest is of very high MW (around 20 million). Do you have any experiences or suggestions on what GPC column we should use?

Answer
I recommend you to consider use of our OHpak SB column series.
This is a polymer (polyhydroxymethacrylate) based size exclusion column suitable for water-soluble polymers.

Please see page 34 on our catalog attached below for more detail.
http://shodex.net/data/downloads/Shodex_Catalog_2008-2010_USA_small.pdf

We have an example of analyzing polyacrylamide using SB-807 column (also on pg 35 of the catalog).
http://shodex.net/index.php?seitenid=1&applic=1079

The exclusion limit of SB-807 is ~500,000,000 (using pullulan as a standard). Thus should meet your requirement.

 
 
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