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question about cleaning column

Record: 776


Category: Specifications
Product: KW
Sample:


Question
So we’ve been experiencing high pressure with the KW-803 set. It seems to me that it’s coming from the guard column because the guard column all by it self at 0.5ml/min is above 200 psi whereas the analytical column by itself at 0.5ml/min is also only slightly above 200 psi. Do you think the guard column is dirty? Overnight reverse rinse in water didn’t help? Do you recommend any other more rigorous ways of cleaning? Or should we just order a new one?

 
 

Answer
Do you know what is the normal backpressure of your analytical column?
It does sound like the problem is guard column though.
Did the pressure increase all the sudden? Did you inject samples different from your regular ones?

You could try washing the guard column, the same way as you would do for analytical column:

 
 
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