S14-0011, 1/2 Shipping Release

Record: 867

Category: Applications
Product: NH2P equilibration
Sample:

Question

Answer
Our tech support in Japan had made the following response:

Please confirm whether the peak is identified as sucrose by MRM.
And please confirm the procedure to make the eluent condition.

We think the peak at 3.7min in Figure 2 might be sucrose.
Though this retention time is earlier than CoA, we think the rate of H2O in the eluent is changed a little by the procedure for making the eluent.
Please refer to the attached file. The retention times are changed by the procedure for making the mixture.

FYI: In the case of LC/MS analysis for sugars, the sensitivity of sugars is improved with using the following eluent condition.
0.05% NH4OH aq./CH3CN = 75/25

Can you please confirm the method you are using to prepare the eluent?

 
 
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