saccharide analysis by SP0810

Record: 677

Category: Specifications
Product: SP
Sample:

Question
I have a question about using RID for CH3CN/water as eluent.
I have been using the Shodex Sugar SP0810 for saccarides analsysis and I can\’t get good separation of arabinose, mannose, and fructose with this column.
The condition that I use is as follows:
Column : Shodex SUGAR SP0810 (8.0mmID*300mm) Eluent : H2O Flow rate : 0.6mL/min Detector : RID from Shimadzu Column temp. : 85deg-C But the arabinose, mannose and fructose are never separated to get good integration and they tend to co-elute.

I found this method to add acetonitrile to the eluent from this site:
http://www.shodex.com/english/dc030277.html
However, the detector used here is the CAD. I would like to know is using RID will also be save for acetonitrile/water combination. I tried 5% acetonitrile before and the detector didn\’t seem to stabilize.

Answer
As you mentioned, unfortunately SP0810 cannot provide baseline separation of those three saccharides.
http://shodex.net/index.php?seitenid=1&applic=625

The reason CAD was used for the acetonitrile method is because it shows rather a large solvent peak.
I am not certain what you mean by “the detector didn’t seem to stabilize”. Are you observing a large baseline noise?
If so, you may want to check the detector.

We do not have a data including fructose in the application.
I am sorry to say, but the separation of those three will probably be very challenging by an isocratic method.
Do you also monitor other saccharide, cellobiose, glucose, xylose, galactose etc?

 
 
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