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SB806M-HQ column

Record: 552


Category: Specifications
Product: SB-806M
Sample:


Question
any recommendation to obtain more accurate MW information for his target substances?

 
 

Answer
Exclusion volume depends on the mobile phase. Sorry I don’t find a good calibration sample to show you this, but below may give you some idea. Please note 2xSB-806M was used for this and exclusion volume was ~ 14 mL.
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=1133
But looking at your chromatogram (symmetrical peak) I think the exclusion volume with your solvent is little over 6 mL.

One example: Separation of 5M and 5KDa dextran sulfate.
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=1063

Based on your elution volumes for the two peaks, actually, you may benefit from even bigger pore sized column (SB-807).
http://shodex.net/index.php?seitenid=1&reiter=r_results&applic=1234
You can see the calibration curve is more “flat” with SB-807 for the elution volumes (7.3 and 8.9 mL).

 
 
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