separation of lysine and methionine. The customer

Record: 28

Category: Column Selection
Product: YS-50
Sample: lysine and methionine

Question
columns for recommendation? Using UV and Fluorescence detectors.

Answer
I think YS-50 is easier to suggest for the analysis of the amino acids.
Please refer to the attached file.
(You can refer this data in Japanese page.
http://www.shodex.com/japanese/dc010914.html )

(See attached file: 100921 amino acids.pdf)

Though lysine is not separated at the same time, we think the elution of lysine may be similar to ornithine, because both lysine and ornithine are basic amino acids.
And I think the retention of lysine is stronger than that of ornithine, because the carbon chain of lysine is longer than ornithine.
So, if the retention of lysine is too strong, we can recommend to use 10mM acetic acid as the eluent as page 2.
(You can refer this data in Japanese page.
http://www.shodex.com/japanese/dc010913.html)

 
 
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