Shodex Columns KW-802.5 and KW-803

Record: 674

Category: Specifications
Product: KW
Sample:

Question
1. Can I run 8M Urea as mobile phase for KW-803 and KW-802.5 columns?

We recommend you to use up to 6 M urea.
Please see page 14 on the operation manual below.
http://shodex.net/data/downloads/PROTEIN_KW-800series_EJ_No.615-5.pdf

2. Does the resolution deteriorate progressively if pH of the mobile phase is greater than pH 7.5? What sort of deterioration do I expect to see at pH greater 7.5?
Does it irreversibly change the column properties ?

Since KW column is a silica base (with surface polymer modification), solvent with pH>7.5 will dissolve the silica and may create void. i.e., yes this is irreversible deterioration.
If you need to use high pH, we would recommend considering SB series GFC column.
http://shodex.net/index.php?seitenid=1&prodcat=22&expd_prod=110

3. Is there a regeneration method for these columns ?

*Cleaning steps for the KW column series
When observing the high pressure, reverse the flow and introduce the eluent at slower flow rate.
When suspecting protein adsorption, use ~0.3M NaCl solution to clean the column.
*do not leave NaCl solution in the column for a long time period since it may cause rust to the column housing.

When suspecting organic substance adsorption, clean with DI water first, and then clean with organic solvent such as MeOH.
Storing the column with 20% MeOH is recommended over sodium azide solution, since NaN3 is a basic eluent and KW series is not recommended to use under basic conditions.

Also for the long term storage, using the following method is the best way.
After cleaning the column with DI water and 20 % MeOH.
1. Remove the exit end of the column from the HPLC system, attach a 1/16 inc 0.8 mm i.d., 50 cm Teflon tubing.
2. Start the pump and once observing eluent flow from the Teflon tubing, stop the pump and place the Teflon tubing in a 100mL solvent resorvoirs which contains 80 mL of the solvent.
3. Remove the entrance side of the column from the HPLC system, screw the end-cap and keep the column (with eluent) at a place with small temperature fluctuations. It is also recommended to change the reserving eluent one a month.

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