Shodex KS-801 Column Cleaning and Regeneration Procedure

Record: 929

Category: Column Cleaning
Product: KS-801
Sample: N/A

Question
What is the recommended cleaning procedure for KS-801 columns?

Answer

For the Shodex SUGAR KS-801 column, cleaning and regeneration are recommended when peak shapes deteriorate, elution times shift, or column pressure increases.

According to the Operation Manual:

Cleaning (for insoluble deposits):

• Reverse the flow direction (introduce eluent from the outlet side).

• Use a flow rate less than half of the recommended operating flow rate.

• Collect the effluent directly to waste (do not pass through detector).

Regeneration (for loss of Na⁺ counter ions):

• Set column temperature to 50 °C.

• Flow rate: 0.5 mL/min.

• Introduce one of the following:

  • Method 1: 50 mL of 0.2 M aqueous sodium hydroxide solution → flush thoroughly with water.

  • Method 2: Flow water at 0.5 mL/min → inject 40 µL of 1 M aqueous sodium hydroxide solution several times → flush thoroughly with water.

⚠ Important Notes:

• Do not connect the detector during regeneration; collect effluent directly to waste.

• Always flush thoroughly with water after using sodium hydroxide.

• Never allow the column to dry.

These steps help restore KS-801 performance when fouling or counter-ion exchange has occurred.

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