Shodex KW column

Record: 544

Category: Column Problem
Product: KW
Sample:

Question
change in retention in the beginning of analysis

Answer
Since the negative peak (probably the solvent peak) observed is also shifting (23->21->19.5 min), shorter elution time may not be only due to the column.
What we suggest you to try are:
1. Check the column-back pressure at the beginning of the analysis (when the elution time is longer) and then check the back pressure again at some time after.
2. Also collect the effluent for a fixed time in the beginning and after several analyses to check if the pump is working properly.

 
 
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