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Shodex SB-802.5 Column for Low Molecular Weight Dextran in 50% DMSO

Record: 933

Category: Column Selection
Product: SB Series
Sample: Dextran

Question
Which column is recommended for analyzing low molecular weight dextran using a 50% DMSO/water eluent?

Answer

For low molecular weight dextran in 50% DMSO/H₂O, the Shodex SB-802.5 column is suitable.

  • The SB series (styrene–divinylbenzene, polymer-based SEC) is compatible with DMSO and mixed aqueous/organic eluents, making it a good choice when other columns (e.g., silica-based SEC) are not stable in these conditions.

  • SB-802.5 provides an exclusion limit of ~20,000 Da, which covers the low MW dextran range effectively.

  • Always ensure the mobile phase is fully miscible and filtered to avoid precipitation, since high concentrations of DMSO can sometimes cause salt or buffer instability.

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