Shodex SI-35 Column for Anion Analysis – Flow Rate and Run Time Optimization

Record: 898

Category: Column Selection
Product: SI-35
Sample: Sulfate

Question
How can the run time for anion analysis on the Shodex SI-35 column be shortened?

Answer

The Shodex SI-35 column can be used for anion detection with typical run times of ~13 minutes at a flow rate of 0.6 mL/min.

• Flow rate: The maximum flow rate is 0.8 mL/min. Increasing the flow slightly can help reduce run time.

• Eluent concentration: Using a higher sodium carbonate concentration can further improve speed.

  • Example: At 4 mM Na₂CO₃ and 0.6 mL/min, sulfate elutes at ~10.7 minutes.

  • At 5 mM Na₂CO₃ and 0.8 mL/min, the run time can be shortened to ~9 minutes.

For faster analysis of common anions, the SI-35 4D column is recommended, offering strong performance with carbonate-based eluents.

Additional Comments

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