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Shodex SP0810 Carbohydrate column performance test

Record: 751


Category: Column Problem
Product: SP0810
Sample:


Question
As I mentioned, I am getting a negative peak at approximately 9.36 min in each and every samples during analysis. Attached the method and chromatogram details of the samples. Please help me to fix the problem as soon as possible. Thank you for your kind help.

 
 

Answer
We think those two negative peaks are oxygen and nitrogen.
Our specialist said she could see small negative peaks in her data also, but not as large as yours..
Would you please try degassing the solvent even though you are using the on-line degasser?
You could do so by using sonicator and aspirator. If this still does not solve the problem, please try the same for your samples.

 
 
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