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Trace Ethylene Glycol (EG) by HPLC — Shodex OHpak SB-802 HQ

Record: 754


Category: Column Selection
Product: SB-802
Sample: ethylene glycol


Question
We need to quantify trace ethylene glycol (EG) (and possibly diethylene glycol, DEG) in an API. Our GC method isn’t performing well. Do you have an HPLC column/detector recommendation?

Answer
Use OHpak SB-802 HQ with RI detection. Shodex provides an application showing EG and DEG separation (referencing USP-NF Polyethylene Glycol 3350) on SB-802 HQ with full conditions and a chromatogram:

Analysis of Ethylene Glycol and Diethylene Glycol in Reference to USP-NF Polyethylene Glycol 3350 (SB-802 HQ)


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