Shodex Equivalent for Agilent ZORBAX Carbohydrate Column (4.6 × 250 mm, 5 µm)

Record: 565

Category: Column Selection
Product: NH2P-50 4E
Sample: N/A

Question
A customer is currently using an Agilent ZORBAX Carbohydrate column (4.6 × 250 mm, 5 µm, P/N 840300-908) and is looking for a Shodex equivalent. What column can be recommended?

Answer
For carbohydrate analysis performed on aminopropyl (NH₂) columns, Shodex NH2P-50 4E is typically the recommended equivalent.

The ZORBAX Carbohydrate column is an aminopropyl silica-based column commonly used under hydrophilic interaction chromatography (HILIC) conditions with high acetonitrile content for the separation of mono- and oligosaccharides.

Shodex NH2P-50 4E provides similar separation characteristics and is designed for saccharide analysis using acetonitrile/water mobile phases with refractive index or evaporative light scattering detection. The polymer-based stationary phase offers improved reproducibility and stability compared to conventional silica-based NH₂ columns.

Method conditions such as mobile phase composition (e.g., high ACN content) can generally be maintained, although minor adjustments may be required to match retention times.

When replacing the column, it is recommended to use the corresponding Shodex NH2P guard column for optimal performance.

Recommended Applications:

• Monosaccharides and Disaccharides (NH2P-50 4E)
• Comparison of Polymer Based and Silica Based Amino Column

Additional Comments

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