HILIC - Polymer Based

Hydrophilic Interaction Liquid Chromatography (HILIC)

Discover the power of Hydrophilic Interaction Liquid Chromatography (HILIC) with our premium range of HILIC columns. Designed for superior separation of polar compounds, our HILIC columns offer unmatched performance and reliability for your analytical needs

Applications:

• Pharmaceutical Analysis: Efficiently separate and analyze polar drug compounds and metabolites
• Biotech Research: Ideal for studying phosphorylated compounds and metabolomics
• Food & Agriculture: Quantify Organic Acids, Sugars, and Vitamins with ease
• Environmental Testing: Excellent retention of polar pesticides like glyphosate and environmental contaminates like short-chain PFAS (ex. TFA)

HILIC Fundamentals

HILIC stands for Hydrophilic Interaction liquid Chromatography. In HILIC mode, a packing material with a highly polar surface is used, with typical functional groups such as amide groups, amino groups, and diol functional groups bonded to silica or polymer base materials. Typically, a polar solvent such as a mixture of water and acetonitrile is used as the eluent, with high a organic solvent ratio to water. Under these conditions, a hydration layer forms on the packing material surface. The analyte’s distribution between the packing material and eluent is influenced by their polarities, analytes are eluted in an order of higher polarity to lower polarity. In HILIC mode, in addition to partitioning, electrostatic interactions, hydrogen bonding, and van der Waals interactions work in a complex manner to achieve high selectivity. Learn more in our "Lesson 3: Separation Modes and their Mechanisms"

HILIC Series Overview

VG-50	Suitable for saccharide analysis using HILIC mode Recovers reducing saccharides with high ratio Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period Easily regenerated by washing in an alkaline solution Appropriate for evaporative light scattering detector, corona charged aerosol detector, and LC/MS
VT-50 2D	Suitable for anionic substances (especially phosphate compounds) analysis using HILIC mode Use of some eluents add ion exchange mode Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period Suitable for LC/MS analysis
VC-50 2D	Modified carboxyl group is suitable for cationic substance analysis including amines The dominant separation mode is RP or IEX rather than HILIC mode
VN-50	The modified diol groups on the packing material create the HILIC mode Suitable for oligonucleotide and oligosaccharide separation which is not possible by SEC or conventional HILIC columns VN-50 1D adopts a double structure housing with an outer surface made of SUS and an inner surface [wetted part] made of PEEK. Covers the weak point of physical strength of PEEK housing and can be used under high pressure conditions. VN-50 1D has an inner diameter of 1.0 mm, which makes it possible to suppress the flow rate to 0.1 mL/min or less, so it suitable for high sensitivity analysis by LC/MS.