Size Exclusion Chromatography
High-Performance SEC Columns for Polymer and Protein Analysis
Researchers in polymer molecular weight analysis and biopharmaceutical characterization need precise, reproducible data for macromolecules and oligomers. Achieving accurate molecular weight and distribution requires size exclusion chromatography columns that deliver reliable performance.
Shodex™ SEC columns directly meet these analytical demands, offering specialized solutions for superior stability and selectivity. Our decades of expertise simplify your SEC challenges, providing consistent, high-performance products that let you confidently advance your macromolecule and oligomer analysis.
Shodex SEC HPLC columns (Size Exclusion Chromatography, also called GPC or Gel Permeation Chromatography) deliver precise molecular weight analysis for polymers, proteins, and macromolecules. Our polymer-based columns provide exceptional resolution from small oligomers to multi-million dalton polymers, with superior pH stability (2-13) for long-term reliability.
Popular applications include protein aggregation testing, antibody characterization, polymer molecular weight distribution, and natural polymer analysis. Choose aqueous SEC columns (PROTEIN KW, OHpak SB) for proteins and water-soluble polymers, or organic GPC columns (KF-800, GPC LF) for synthetic polymers in THF or chloroform.
Need help selecting the right SEC HPLC column for your application? Contact our technical team for expert method development support.
| Characteristics of Size Exclusion Chromatography |
|---|
| Network of pores on the surface of the packing material works as molecular sieve to separate molecules based on hydrodynamic size. |
| To separate molecules solely based on hydrodynamic size (molecular size in solution), it requires an analytical condition that prevents interactions between compounds and packing gel. |
| The bigger the molecule size, the faster the elution sequence. |
| Used for molecular weight or molecular distribution determination of macromolecules and characterization of oligomers. |
| SEC is a non-interactive separation technique; minimizing interactions between analytes and the stationary phase is critical for accurate molecular weight determination. |
Specialized SEC Columns for Every Application
Shodex offers size exclusion chromatography columns tailored to meet the diverse needs of modern analytical laboratories, helping researchers identify the optimal solution for their specific soluble samples.
Gel Filtration Chromatography (GFC)
Our GFC columns are specifically engineered to separate water-soluble samples by size, making them ideal for aqueous SEC columns. Choose from our durable polymer-based GFC or specialized silica-based GFC options. These silica-based columns excel in applications such as protein SEC columns and precise monoclonal antibody SEC analysis, providing high-resolution separations for complex biological mixtures and ensuring the reproducibility critical for biopharmaceutical characterization. Our polymer based GFC column excel with accurate moleclar wieght determination of polar polymers like polysaccharides.
Gel Permeation Chromatography Columns (GPC)
For laboratories working with organic-soluble samples, our GPC columns provide robust solutions. Compatible with solvents like THF, DMF, and Chloroform, our organic GPC columns are well-suited for analyzing the molecular weights of complex polymers, delivering reliable data for both synthetic and natural polymers.
Multimode and Multi-solvent GPC
Shodex’s versatile multimode and multi-solvent GPC offerings are designed to handle a broader range of challenging samples with multiple solvent options for flexible polymer analysis. These GPC columns provide exceptional flexibility and efficiency for complex analyses where a single solvent system is insufficient.
Why Trust Our Chromatography Solutions?
Shodex stands apart by combining material durability, expertise and superior logistics, addressing the critical needs of analytical laboratories and quality control departments. Benefits of working with us include:
- Polymer-based durability: Shodex specializes in polymer-based GFC and GPC columns, offering superior long-term durability, extended column lifespan and exceptional cost-effectiveness compared to generic silica alternatives.
- Decades of SEC specialization: We are a deeply specialized pioneer with over five decades of expertise in SEC columns, backed by vast, publicly accessible methodologies.
- Logistical expertise: Benefit from fast delivery and local expert support for your molecular weight analysis needs anywhere in the Americas.
Upgrade Your Analytical Capabilities With Shodex
Experience the benefits of durable, cost-effective SEC columns backed by direct logistical support and a legacy of polymer expertise. Browse our specific product categories above, or contact the Shodex technical team for recommendations on custom SEC columns.
High-Performance SEC Columns for Polymer and Protein Analysis
Researchers in polymer molecular weight analysis and biopharmaceutical characterization need precise, reproducible data for macromolecules and oligomers. Achieving accurate molecular weight and distribution requires size exclusion chromatography columns that deliver reliable performance. Shodex™ SEC columns directly meet these analytical demands, offering specialized solutions for superior stability and selectivity. Our decades of expertise simplify your SEC challenges, providing consistent, high-performance products that let you confidently advance your macromolecule and oligomer analysis.
Shodex SEC HPLC columns (Size Exclusion Chromatography, also called GPC or Gel Permeation Chromatography) deliver precise molecular weight analysis for polymers, proteins, and macromolecules. Our polymer-based columns provide exceptional resolution from small oligomers to multi-million dalton polymers, with superior pH stability (2-13) for long-term reliability.
Popular applications include protein aggregation testing, antibody characterization, polymer molecular weight distribution, and natural polymer analysis. Choose aqueous SEC columns (PROTEIN KW, OHpak SB) for proteins and water-soluble polymers, or organic GPC columns (KF-800, GPC LF) for synthetic polymers in THF or chloroform.
Need help selecting the right SEC HPLC column for your application? Contact our technical team for expert method development support.
| Characteristics of Size Exclusion Chromatography |
|---|
| Network of pores on the surface of the packing material works as molecular sieve to separate molecules based on hydrodynamic size. |
| To separate molecules solely based on hydrodynamic size (molecular size in solution), it requires an analytical condition that prevents interactions between compounds and packing gel. |
| The bigger the molecule size, the faster the elution sequence. |
| Used for molecular weight or molecular distribution determination of macromolecules and characterization of oligomers. |
| SEC is a non-interactive separation technique; minimizing interactions between analytes and the stationary phase is critical for accurate molecular weight determination. |
Specialized SEC Columns for Every Application
Shodex offers size exclusion chromatography columns tailored to meet the diverse needs of modern analytical laboratories, helping researchers identify the optimal solution for their specific soluble samples.Gel Filtration Chromatography (GFC)
Our GFC columns are specifically engineered to separate water-soluble samples by size, making them ideal for aqueous SEC columns. Choose from our durable polymer-based GFC or specialized silica-based GFC options. These silica-based columns excel in applications such as protein SEC columns and precise monoclonal antibody SEC analysis, providing high-resolution separations for complex biological mixtures and ensuring the reproducibility critical for biopharmaceutical characterization. Our polymer based GFC column excel with accurate moleclar wieght determination of polar polymers like polysaccharides.Gel Permeation Chromatography Columns (GPC)
For laboratories working with organic-soluble samples, our GPC columns provide robust solutions. Compatible with solvents like THF, DMF, and Chloroform, our organic GPC columns are well-suited for analyzing the molecular weights of complex polymers, delivering reliable data for both synthetic and natural polymers.Multimode and Multi-solvent GPC
Shodex's versatile multimode and multi-solvent GPC offerings are designed to handle a broader range of challenging samples with multiple solvent options for flexible polymer analysis. These GPC columns provide exceptional flexibility and efficiency for complex analyses where a single solvent system is insufficient.Why Trust Our Chromatography Solutions?
Shodex stands apart by combining material durability, expertise and superior logistics, addressing the critical needs of analytical laboratories and quality control departments. Benefits of working with us include:- Polymer-based durability: Shodex specializes in polymer-based GFC and GPC columns, offering superior long-term durability, extended column lifespan and exceptional cost-effectiveness compared to generic silica alternatives.
- Decades of SEC specialization: We are a deeply specialized pioneer with over five decades of expertise in SEC columns, backed by vast, publicly accessible methodologies.
- Logistical expertise: Benefit from fast delivery and local expert support for your molecular weight analysis needs anywhere in the Americas.