Lesson 1: Introduction to HPLC
1. Introduction
2. What is liquid chromatography?
3. What is HPLC?
4. Components of HPLC
4.1 Pump
4.2 Injector
4.3 Column
4.4 Detector
4.5 Detector/Recorder
4.6 Degasser
4.7 Column heater
5. Other chromatography
Lesson 2: Theory and types of HPLC column
1. Theory of LC column Separation
2. HPLC separation
3. Types of packed Gels
3.1 Silica Gel
3.2 Polymer Gel
3.3 Other Gel
4. Types of Separation Mode
Lesson 3: Partition/Adsorption Chromatography
1. Reversed-Phase Column
1.1 ODS column
1.2 Other Silica-Based Columns
1.3 Polymer-Based Columns
2. Normal-Phase Column
3. Hydrophilic Interaction Chromatography (HILIC) Column
4. Summary of Shodexâ„¢ Partition/Adsorption Columns
Lesson 4: SEC Columns
1. Size Exclusion Columns
1.1 Theory of SEC mode
1.2 Molecular Distribution Analysis
1.3 Separation Based on SEC
1.4 Types of SEC
2. Gel Filtration Chromatography
3. Gel Permeation Chromatography
4. Linear Column
5. Downsized Column
6. Analysis of Polymers that require Special Conditions
6.1 High Temperature GPC
6.2 HFIP
7. Multi-Mode Column
8. Multi-Solvent Column
Lesson 5: Other Separation Modes, Preparative and Guard Columns
1. Ion Exchange Column
2. Ion Chromatography Column
2.1 Suppressor Type Ion Chromatography Column
2.2 Non-Suppressor Type Ion Chromatography Column
2.3 Other Columns
3. Ion Exclusion Column
4. Ligand Exchange Column
5. Affinity Column
6. Chiral Column
7. Special Use Columns
7.1 Pesticides (Used at Golf Course) Analysis Column
7.2 Pesticides (N-Methylcarbamate) Analysis Column
7.3 Surfactant Removal Column
7.4 Amino Acid Analysis Column
7.5 Cyan Analysis Column
7.6 Mannitol Analysis Column
7.7 <em>Umami</em> Components Analysis Column
7.8 Sample Clean-up Column
8. Preparative Column
9. Guard Column
Lesson 6: Detectors for HPLC
1. UV, VIS, and PDA Detectors
2. Refractive-Index Detector
3. Evaporative Light Scattering Detector
4. Multi-Angle Light Scattering Detector
5. Mass Spectrometer
6. Conductivity Detector
7. Fluorescence Detector
8. Chemiluminescence Detector
9. Optical Rotation Detector
10. Electro Chemical Detector
Lesson 7: Ion Chromatography
Webinar 1: Shodex HPLC Column Selection Based on Sample Characteristics
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